Team:KU Leuven
From 2013.igem.org
Secret garden
Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!
- A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
- For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
- We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?
Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!
E. coligy: Plants with BanAphids
Since the world population keeps on growing and all people need to be fed, it’s important that crop yields are optimized. Different factors ranging from weather to diseases and pests have a huge impact on food supplies worldwide. An example of one of these factors are aphid pests, these can lead to yield losses worth millions of dollars! Aphids, little insects belonging to the family of Aphididae, can be found everywhere: ranging from crop fields to fruit trees and even in vegetable and flower gardens. Farmers try to control them by using insecticides, but these harm the environment and aphids can become resistant to them quite rapidly. This is why we, the KU Leuven iGEM 2013 team, created an ecological insecticide-free aphid controlling mechanism, which mimics natural signalling systems used by both plants and insects.
Our bacteria are called BanAphids and produces two pheromones. The first one is methylsalicylate, which plays a role in a plant’s defence response and is produced by the plant upon infestation. The second pheromone is β-farnesene, which is an aphid alarm pheromone. In our system the BanAphids produce β-farnesene to repel aphids, while the methylsalicylate is meant to attract their natural predators such as ladybugs. We proved the functionality of these pheromones by performing insect experiments at two companies, Biobest and PC Fruit. Both companies were very interested in the concept of our project.
We designed two systems in which E. coligy can be incorporated. In the first system bacteria are sprayed on the plants and in this case the production of pheromones is dependent on the presence of aphids. This system avoids the habituation of aphids by only becoming active in the presence of aphids. The second system consists of a sticker containing E. coligy, that can be attached to a plant. In this system the production of both pheromones is alternated by the use of our own designed oscillating system. This oscillator was very extensively modelled by our team.
Glucose model
This drawing shows our BanAphid producing EBF and MeS to get rid of the aphids and attract a ladybug.
"Sticker" or "Oscillator" model
This drawing shows how the sticker could be placed in a rose bush to repel aphids and attract ladybugs.
Since E. coligy will be introduced in the environment, the ethical aspect of our project cannot be underestimated. Therefore a philosophy student joined our team to study the link between ethics and synthetic biology. He did this by interviewing the complete team, to clarify our ethical beliefs and their relation to synthetic biology and we organized a lecture by and meeting with Hub Zwart (a Dutch philosopher focusing on science). Finally, we wrote an ethical evaluation of our project.
We organized a symposium and debate to inform the general public about our project and to open the discussion on the ethical aspects. We invited our neighbouring Benelux iGEM teams for both events. Some Benelux iGEM-teams participated this year so we are now contemplating a "Benelux-GEM slam" for the future. We also went out on the street and interviewed farmers to ask if they would use E. coligy and did several school visits with our own biobricks and 3D-bacterium design to let students take their first steps in the fascinating world of synthetic biology.
Video