Team:DTU-Denmark/Notebook/9 July 2013
From 2013.igem.org
Contents |
208
Main purpose
Run gel with 7 Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.
Purification of Nir operon and Nanodrop measurement.
Who was in the lab
Ariadni,,Henrike,Julia
Procedure
Run gel:
- 1ul of dye
- 5ul of sample
- 5ul Kb ladder
Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)
PCR
Results
Nanodrop measurement:
- ng/ul (Nir)
- ng/ul (Nir)
- ng/ul (Nir)