Team:DTU-Denmark/Notebook/9 July 2013

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Contents

208

Main purpose

Run gel with 7 Nir operon from Pseudomonas aeruginosa and purified AMO, HAO and CYC from 08.07.2013.

Purification of Nir operon and Nanodrop measurement.

Who was in the lab

Ariadni,,Henrike,Julia

Procedure

Run gel:

  • 1ul of dye
  • 5ul of sample
  • 5ul Kb ladder

Purification of Nir DNA according to the protocol QIA purification protocol. (given in the Kit)

PCR

Results

Nanodrop measurement:

  • ng/ul (Nir)
  • ng/ul (Nir)
  • ng/ul (Nir)