"
Team:UNITN-Trento/Notebook/Labposts/08/41
From 2013.igem.org
{"date" : "2013-08-18","author" : "thomas","title" : "Preparing sequencing samples of AraC-pBAD EFE","content" : "This morning I got to prepares samples of AraC-pBAD EFE to send them for sequencing. We decided to sequence both samples with Vf and Vr primers. So knowing the preps concentrations, I prepared the mix as follow:
Quantification results
The samples were then putted in a falcon and send with a courier. ","tags" : "aracpbad-efe"}
| Volume added to sample 1:1 (µl) | Volume added to sample 1:2 (µl) | |
|---|---|---|
| Template DNA | 5.5 | 6.5 |
| Primer | 2 | 2 |
| Water | up to 15 | up to 15 |
