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From 2013.igem.org

Labbook |	Protocols |	Materials |

The following page gives an overview on the generalized protocols and their possible modifications that where used our 2013 iGEM project. The corresponding materials can be found in the materials section.

Contents 1 In vivo 1.1 [http://en.wikipedia.org/wiki/E._coli Escherichia coli] 2 In vitro 2.1 [http://en.wikipedia.org/wiki/DNA DNA] 2.2 [http://en.wikipedia.org/wiki/Protein Protein]

In vivo

[http://en.wikipedia.org/wiki/E._coli Escherichia coli]

• Bacterial cell culture
• Bacterial transformation
• Cell counting/plating
• Chemically competent cells
• Colony PCR
• Glycerine Stocks
• Heat Shock Transformation
• Protein expression of LssmOrange and mKate

In vitro

[http://en.wikipedia.org/wiki/DNA DNA]

• Agarose gel electrophoresis
• Dephosphorylation
• DNA Ligation
• DNA Quantification with NanoDrop
• InFusion cloning
• PCR
• PureYield Plasmid Miniprep System
• Restriction digest
• [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing of DNA]
• Wizard SV Gel and PCR Clean-Up System

[http://en.wikipedia.org/wiki/Protein Protein]

• SDS-Page
• Spectra measurement