Team:Heidelberg/Templates/MM week13p overview

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Goal

File:PIK1.svg
Vector map of the pIK1 plasmid.
File:PIK2.svg
Vector map of the pIK2 plasmid.

Back-up plan in case the genomic integration does not work. Two plasmids are to be constructed, pIK1 (containing BBa_I746200, pccB-accA, and sfp) and pIK2 (containing pccB-accA and sfp) in the pSB3C5 backbone. 6 fragments are to be amplified:

fragment ID source primers size [kb]
f1 BBa_J04450 in pSB3C5 IK32+IK33 3
f2 BBa_I746200 IK26+IK27 2.3
f3 pccB-accA in pLF03 IK38+IK29 3.4
f4 sfp in BAP1 IK30+IK31 0.7
f5 BBa_J04450 in pSB3C5 IK32+IK35 3
f6 pccB-accA in pLF03 IK34+IK29 3.4
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