Team:Tuebingen/Notebook/Protocols/lb

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LB Medium
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Reagents

10.0 g Trypton
5.0 g Yeast extract
10.0 g NaCl
Antibiotics

 

Procedure

Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. Let cool down (LB-medium stays liqud) and add antibiotics if required. Store at 4 °C.


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