Team:Tuebingen/Notebook/Protocols/tae
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TAE Buffer
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Reagents
242.0 g | Tris Base |
57.1 mL | Glacial acetic acid |
100 mL | EDTA (c = 0.5 M ; pH 8.0) |
10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature.
Procedure yields 50x concentration.