Team:Tuebingen/Notebook/Protocols/glycerol-stocks

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Procedure

1. Inoculate 5 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
2. On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.
3. Add 1 mL of cell-culture and mix.