Team:Groningen/Labwork/12 July 2013

From 2013.igem.org

Mirjam Made a PCR with (1) no strep tag (2) strep F (3) strep R. The annealing temperature for (1) and (2) is 78 degrees Celsius. For (3) the annealing temperature is 80 degrees Celsius.

Gel purification of the strep F silk obtained yesterday.
Run a 0.8% agarose gel for 30 min at 90V.

Inne
Made 3 bottles of 250 ml LB Agar (20g/L LB, 16g/L Agar)
Autoclaved:
3 bottles of demiwater(80 ml)
3 bottles of tabwater (80 ml)
3 bottles of miliQ (80 ml)
3 bottles of LB Agar
1 bottle of miliQ (1 L)