"
Team:Heidelberg/Templates/Materials list
From 2013.igem.org
Materials used during the project
Partly adopted from iGEM Team Heidelberg 2009 and 2010. Please recheck.
Table 1: Kits
| Kit | Supplier | Catalog Number |
|---|---|---|
| QIAquick® Gel Extraction Kit (250) | QIAGEN | 28706 |
| QIAquick® Nucleotid Removal Kit (250) | QIAGEN | 28306 |
| QIAEX II® Gel Extraction Kit (500) | QIAGEN | 20051 |
| MinElute® PCR Purification Kit (250) | QIAGEN | 28006 |
| QIAquick® PCR Purification Kit (250) | QIAGEN | 28106 |
| QIAGEN® Plasmid Plus Midi Kit (100) | QIAGEN | 12945 |
| QIAprep® Spin Miniprep Kit (250) | QIAGEN | 27106 |
| Plasmid Plus Maxi Kit (25) | QIAGEN | 12963 |
| Plasmid Plus Midi Kit (25) | QIAGEN | 12943 |
| QIAprep Spin Miniprep Columns | QIAGEN | 27115 |
Table 2: Marker
| Marker | Supplier | Catalog Number |
|---|---|---|
| Quick-Load 2-Log DNA Ladder (0.1-10.0 kb) | New England BioLabs | N3200S |
| Quick-Load® 1 kb DNA Ladder | New England BioLabs | N0468S |
| 50 bp DNA Ladder | New England BioLabs | N3236S |
| Gel loading solution | Sigma-Aldrich Chemie GmbH | G2526-5ML |
Table 3: Enzymes
| Enzyme | Supplier | Catalog Number |
|---|---|---|
| EcoRI | New England BioLabs | |
| NotI-HF | New England BioLabs | R3189 S |
| PstI | New England BioLabs | |
| PstI-HF | New England Biolabs | R3140 S |
| SpeI-HF | New England BioLabs | R3133 S |
| XbaI | New England BioLabs | R0145 S |
| PvuI-HF | New England BioLabs | R3150S |
| NheI-HF | New England BioLabs | R3131 S |
| KpnO-HF | New England BioLabs | |
| Phusion Flash High-Fidelity PCR MAster Mix | Biozym Scientific GmbH | F-548L |
| Phusion® High-Fidelity PCR Master mix | New England Biolabs GmbH | M0531 L |
| Gibson Assembly# Master Mix | New England Biolabs GmbH | E2611 S |
| Lambda DNA Mono Cut Mix | New England Biolabs GmbH | N3019 S |
| T4 DNA Ligase | New England Biolabs GmbH | M0202 S |
| PacI, recombinant | New England Biolabs GmbH | R0547 S |
| EcoRI-HF | New England Biolabs GmbH | R3101 S |
| SalI-HF | New England Biolabs GmbH | R3138 S |
| NotI-HF | New England Biolabs GmbH | R3189 S |
| SpeI-HF | New England Biolabs GmbH | R3133 S |
| 2x PCR Master mix Solution (iTaq) | HISS DIAGNOSTICS GmbH | 25028 |
| DpnI | New England Biolabs GmbH | R0176 S |
| KpnI-HF | New England Biolabs GmbH | R3142 S |
| Calcium Carbonate Bioxtra | Sigma-Aldrich Chemie GmbH | C4830-100G |
| BamI | New England Biolabs GmbH | R3136 S |
| HindIII-HF | New England Biolabs GmbH | R3104 S |
| Dream Taq PCR MM | Fermentas Life Sciences | K1071 |
| Lysozyme from chicken egg white | Sigma-Aldrich Chemie GmbH | L4919-500MG |
| Pronasw E from Streptomyces griseus | Sigma-Aldrich Chemie GmbH | P6911-100MG |
| BgIII | New England Biolabs GmbH | R0144 S |
| Gibson Assembly# Master Mix | New England Biolabs GmbH | E2611 S |
| MfeI-HF | New England Biolabs GmbH | R3589 S |
| DreamTaq Green PCR Master Mix (2X) | Thermo FisherScientificBiosciences GmbH | K1081 |
Table 4: Bacteria
| Strain | Supplier | Catalog Number |
|---|---|---|
| DH10beta | ||
| Top10 | ||
| BapI | ||
| Delftia acidovorans | DSMZ | DSM39 |
| Streptomyces lavendulae lavendulae | DSMZ | DSM40708 |
| Streptomyces mobaraensis | DSMZ | DSM 40903 |
| Photorhabdus laumondii luminescens | DSMZ | DSM 15139 |
| Delftia acidovorans | DSMZ | DSM 14801 |
Table 5: Media and Antibiotics
| Antibiotic | Supplier | Catalog Number | Final Concentration↓ | Solvent |
|---|---|---|---|---|
| Ampicillin anhydrous crystalline | Sigma-Aldrich Chemie GmbH | A9393-5G | 1:1000 | Ethanol |
| Ampicillin sodium crystalline | A9518-5G | 1:1000 | Ethanol | |
| Chloramphenicol crystalline | Sigma-Aldrich Chemie GmbH | C0378-5G | 1:3000 | Ethanol |
| Kanamycinsulfat Gemisch der Komponentena | Sigma-Aldrich Chemie GmbH | 60615-5G | 1:1000 | H2O |
| Tetracycline | ||||
| SOC Outgrowth Medium | New England Biolabs GmbH | B9020 S | ||
| LB BROTH BASE | Th. Geyer GmbH & Co KG | SA/L3022/001000 | 20g/1l | H2O |
| LB Broth powder | Sigma-Aldrich Chemie GmbH | L3022-1KG | ||
| Agarose molecular Biology Reagent | Th. Geyer GmbH & Co KG | SA/A9539/000050 | 0,5% | H2O |
| Propionic acid sodium insect cell*culture | Sigma-Aldrich Chemie GmbH | P5436-100G | ||
| Agarose for routine use | Sigma-Aldrich Chemie GmbH | A9539-100G | ||
| Bacitracin | Sigma-Aldrich Chemie GmbH | B0125-50KU |
preparation of M9 minimal media according to Serva company
Table 6: Primers and Oligos
| Oligo | Supplier | Catalog Number |
|---|---|---|
| BpsA_TE_Stop_8_rbs_6_rv | Sigma-Aldrich Chemie GmbH | VC00021'20637090 |
| pSB1C3_BBa_J04450_rv | Sigma-Aldrich Chemie GmbH | VC00021`20637084 |
| pSB1C3_BBa_J04450_fw | Sigma-Aldrich Chemie GmbH | VC00021`20637083 |
| svp_cds_fw | Sigma-Aldrich Chemie GmbH | VC00021`20637091 |
| BpsA_AOxA_rv | Sigma-Aldrich Chemie GmbH | VC00021`20637086 |
| BpsA_TE_fw | Sigma-Aldrich Chemie GmbH | VC00021`20637089 |
| BpsA_T_rv | Sigma-Aldrich Chemie GmbH | VC00021`20637088 |
| BpsA_AOxA_fw | Sigma-Aldrich Chemie GmbH | VC00021`20637085 |
| BpsA_T_fw | Sigma-Aldrich Chemie GmbH | VC00021`20637087 |
| svp_cds_rv | Sigma-Aldrich Chemie GmbH | VC00021`20637092 |
| IK36:pLF03_seq_rev | Sigma-Aldrich Chemie GmbH | VC00021`21393236 |
| IK37:pLF03-catR_seq_rev | Sigma-Aldrich Chemie GmbH | VC00021`21393237 |
| SR_01_FS34_fwtoFS26 | Sigma-Aldrich Chemie GmbH | VC00021`21457452 |
| FS_27_OP_rv | Sigma-Aldrich Chemie GmbH | VC00021`21457453 |
| FS_28_OP_rv | Sigma-Aldrich Chemie GmbH | VC00021`21457454 |
| FS_29_OP_rv | Sigma-Aldrich Chemie GmbH | VC00021`21457455 |
| FS_30_OP_rv | Sigma-Aldrich Chemie GmbH | VC00021`21457456 |
| FS_31_OP_fw | Sigma-Aldrich Chemie GmbH | VC00021`21457457 |
| FS_32_OPgood_fw | Sigma-Aldrich Chemie GmbH | VC00021`21457458 |
| FS_33_OP_fw | Sigma-Aldrich Chemie GmbH | VC00021`21457459 |
| RB46:indC-woPPT_rv | Sigma-Aldrich Chemie GmbH | VC00021`21487465 |
| RB47:indC-SpeI_fw | Sigma-Aldrich Chemie GmbH | VC00021`21487466 |
| RB48:indC-EcoRI_rv | Sigma-Aldrich Chemie GmbH | VC00021`21487467 |
| RB49:indC-EcoRI_fw | Sigma-Aldrich Chemie GmbH | VC00021`21487468 |
| RB50:indC-SpeI_rv | Sigma-Aldrich Chemie GmbH | VC00021`21487469 |
| RB51:pSB(2/3)K3_fw | Sigma-Aldrich Chemie GmbH | VC00021`21487470 |
| RB52:pSB(2/3)K3_rv | Sigma-Aldrich Chemie GmbH | VC00021`21487471 |
| RB57:tycC6-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522074 |
| RB58:tycC6-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522075 |
| RB56:tycA1-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522073 |
| RB55:tycA1-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522072 |
| RB53:entF-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522070 |
| RB54:entF-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522071 |
| RB67:DelC-pSB2K3_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522084 |
| RB66:DelC-pSB2K3_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522083 |
| RB63:pSB(2/3)K3_rv_korr | Sigma-Aldrich Chemie GmbH | VC00021`21522080 |
| RB65:DelC-extr_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522082 |
| RB59:delH4-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522076 |
| RB60:delH4-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522077 |
| RB61:delH5-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522078 |
| RB62:delH5-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522079 |
| RB64:DelC-extr_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522081 |
| KH9:ccdB-Big_fw | Sigma-Aldrich Chemie GmbH | VC00021`21522085 |
| KH10:ccdB-Big_rv | Sigma-Aldrich Chemie GmbH | VC00021`21522086 |
| AT08:RFC10suffix_TycC6_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564137 |
| KH13_BBa-entD_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564138 |
| AT03:RFC10prefix-TycB1_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564132 |
| AT06:RFC10suffix_TycC5_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564135 |
| AT02:RFC10suffix_TycA_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564131 |
| AT01:RFC10prefix_TycA_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564130 |
| AT05:RFC10prefix-TycC5_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564134 |
| AT07:RFC10prefix-TycC6_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564136 |
| AT04:RFC10suffix_TycB1_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564133 |
| KH21_BBa-indC_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564146 |
| KH20_BBa-delC_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564145 |
| KH22_BBa-indC_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564147 |
| KH15_BBa-sfp_Bsub_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564140 |
| KH16_BBa-sfp_Bsub_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564141 |
| KH17_BBa-svp_Svert_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564142 |
| KH14_BBa-entD_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564139 |
| KH18_BBa-svp_Svert_rv | Sigma-Aldrich Chemie GmbH | VC00021`21564143 |
| KH19_BBa-delC_fw | Sigma-Aldrich Chemie GmbH | VC00021`21564144 |
| KH13_BBa-entD_fw | Sigma-Aldrich Chemie GmbH | VC00021`21566979 |
| KH14_BBa-entD_rv | Sigma-Aldrich Chemie GmbH | VC00021`21566980 |
| NK_01_FS_62_screen_nomRFP_rv | Sigma-Aldrich Chemie GmbH | VC00021`21599362 |
| FS_46_L_rv_gibsontoBBpSB4K5 | Sigma-Aldrich Chemie GmbH | VC00021`21599355 |
| FS_61_screening_with_mRFP | Sigma-Aldrich Chemie GmbH | VC00021`21599361 |
| FS_52_screening_AF_FG21_26_rv | Sigma-Aldrich Chemie GmbH | VC00021`21599359 |
| FS_51_screening_AF_FG21_26_fw | Sigma-Aldrich Chemie GmbH | VC00021`21599358 |
| FS_45_pSB4K5_fw_wo_mRFP | Sigma-Aldrich Chemie GmbH | VC00021`21599354 |
| FS_60_screening_L_BB_fw | Sigma-Aldrich Chemie GmbH | VC00021`21599360 |
| FS_48_screening_BB_AF_rv | Sigma-Aldrich Chemie GmbH | VC00021`21599357 |
| FS_47_screening_BB_AF_fw | Sigma-Aldrich Chemie GmbH | VC00021`21599356 |
| AT05_RFC10prefix_TycC5_fw | Sigma-Aldrich Chemie GmbH | VC00021`21599363 |
| AT07_RFC10prefix_TycC6_fw | Sigma-Aldrich Chemie GmbH | VC00021`21599364 |
| PW17:TycAdE-C(TycC2)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21632288 |
| PW15:C(TycC2)-indC_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632286 |
| PW14:C(TycC2)-indC_rev | Sigma-Aldrich Chemie GmbH | VC00021`21632285 |
| PW16:indC_rev | Sigma-Aldrich Chemie GmbH | VC00021`21632287 |
| KH11_plu2642-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21632281 |
| RB77:plu2670-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21632284 |
| KH12_plu2642-T_rv | Sigma-Aldrich Chemie GmbH | VC00021`21632282 |
| RB76:plu2670-T_fw | Sigma-Aldrich Chemie GmbH | VC00021`21632283 |
| PW18:TycAdE-C(TycC2)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632289 |
| PW19:TycC4dC_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632290 |
| PW20:TycC4dC-C(TycC2)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21632291 |
| PW21:TycC4dC-C(TycC2)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632292 |
| PW22:pSB1C3-TycC4dC_rev | Sigma-Aldrich Chemie GmbH | VC00021`21632293 |
| PW23:indC-pSB1C3_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632294 |
| PW24:TycC1dC_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21632295 |
| PW25:pSB1C3-TycC1dC | Sigma-Aldrich Chemie GmbH | VC00021`21632296 |
| HM13:Screen_DelH_end_fw | Sigma-Aldrich Chemie GmbH | VC00021`21635842 |
| HM14:DelH_tetR_fw | Sigma-Aldrich Chemie GmbH | VC00021`21635843 |
| HM15:tetR_stop_BB_rev | Sigma-Aldrich Chemie GmbH | VC00021`21635844 |
| HM16:tetR_pSB6A1_fw | Sigma-Aldrich Chemie GmbH | VC00021`21635845 |
| HM17:DelH_Terminator_BB_fw | Sigma-Aldrich Chemie GmbH | VC00021`21635846 |
| FS_63_rv_scre_mRFP_to_DelL | Sigma-Aldrich Chemie GmbH | VC00021'21714285 |
| FS_57_rv_scre_DelOP_to_DelG | Sigma-Aldrich Chemie GmbH | VC00021'21714286 |
| FS_58_fw_scre_DelOP_to_DelL | Sigma-Aldrich Chemie GmbH | VC00021'21714287 |
| AT09_R10_B1_fw_longer | Sigma-Aldrich Chemie GmbH | VC00021`21736751 |
| AT10_R10_B1_rv_longer | Sigma-Aldrich Chemie GmbH | VC00021`21736752 |
| AT11_R10_C5_fw_rpos | Sigma-Aldrich Chemie GmbH | VC00021`21736753 |
| RB78_indC_SpeI_rv_end | Sigma-Aldrich Chemie GmbH | VC00021`21736755 |
| FS_66_DelH4_rv | Sigma-Aldrich Chemie GmbH | VC00021`21736757 |
| FS_68_DelH6_rv | Sigma-Aldrich Chemie GmbH | VC00021`21736759 |
| FS_67_DelH5_fw | Sigma-Aldrich Chemie GmbH | VC00021`21736758 |
| FS_65_DelH3_fw | Sigma-Aldrich Chemie GmbH | VC00021`21736756 |
| FS_64_DelH2_rv | Sigma-Aldrich Chemie GmbH | VC00021`21736754 |
| FS_69_DelH7_fw | Sigma-Aldrich Chemie GmbH | VC00021`21736760 |
| FS_70_DelH8_rv | Sigma-Aldrich Chemie GmbH | VC00021`21736761 |
| FS_71_NK_02_DelH9_fw | Sigma-Aldrich Chemie GmbH | VC00021`21736762 |
| RB79_indC_woPPTase_rv2 | Sigma-Aldrich Chemie GmbH | VC00021`21784357 |
| IK38:pIK1_fw | Sigma-Aldrich Chemie GmbH | VC00021`21792233 |
| IK39:pIK1_rev | Sigma-Aldrich Chemie GmbH | VC00021`21792234 |
| FS_76_SR_06_rv | Sigma-Aldrich Chemie GmbH | VC00021`21806264 |
| FS_72_SR_02_fw | Sigma-Aldrich Chemie GmbH | VC00021`21806265 |
| FS_73_SR_03_fw | Sigma-Aldrich Chemie GmbH | VC00021`21806266 |
| FS_74_SR_04_fw | Sigma-Aldrich Chemie GmbH | VC00021`21806267 |
| FS_75_SR_05_fw | Sigma-Aldrich Chemie GmbH | VC00021`21806268 |
| PW33:TycC6dTE-C(TycC2)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841357 |
| PW34:TycC6dTE-C(TycC2)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841358 |
| PW27:TycC5dC-C(TycC4)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841351 |
| PW28:TycC5dC-C(TycC4)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841352 |
| PW29:C(TycC4)-TycC4dC_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841353 |
| PW30:C(TycC4)-TycC4dC_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841354 |
| PW31:TycC4-C(TycC4)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841355 |
| PW32:TycC4-C(TycC4)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841356 |
| PW26:TycC5dC_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841350 |
| PW35:TycC6dTE-C(TycC4)_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841359 |
| PW36:TycC6dTE-C(TycC4)_fwd | Sigma-Aldrich Chemie GmbH | VC00021`21841360 |
| PW37:pSB1C3-TycC5dC_rev | Sigma-Aldrich Chemie GmbH | VC00021`21841361 |
| AT101 | Sigma-Aldrich Chemie GmbH | VC00021`21865217 |
| AT102 | Sigma-Aldrich Chemie GmbH | VC00021`21865218 |
| AT103 | Sigma-Aldrich Chemie GmbH | VC00021`21865219 |
| AT104 | Sigma-Aldrich Chemie GmbH | VC00021`21865220 |
| AT105 | Sigma-Aldrich Chemie GmbH | VC00021`21865221 |
| AT106 | Sigma-Aldrich Chemie GmbH | VC00021`21865222 |
| AT107 | Sigma-Aldrich Chemie GmbH | VC00021`21865223 |
| AT108 | Sigma-Aldrich Chemie GmbH | VC00021`21865224 |
| IK40:pIK2-BBa_J23114 | Sigma-Aldrich Chemie GmbH | VC00021`21871657 |
| IK41:BBa_I746200-Bba | Sigma-Aldrich Chemie GmbH | VC00021`21871658 |
| IK42:BBa_I746200_rev | Sigma-Aldrich Chemie GmbH | VC00021`21871659 |
| IK43:pIK2-BBa_I746200_fw | Sigma-Aldrich Chemie GmbH | VC00021`21871660 |
| HM20_BB-pSB6A1_ | Eurofins MWG GmbH | 27080132 |
| FS_77_rv | Eurofins MWG GmbH | 27080134 |
| NI19:ngrA-BB_fw | Eurofins MWG GmbH | 27114029 |
| NI20:ngrA_rv | Eurofins MWG GmbH | 27114030 |
| NI21:ngrA_fw | Eurofins MWG GmbH | 27114031 |
| NI22:ngrA_rv | Eurofins MWG GmbH | 27114032 |
| VF2 | Eurofins MWG GmbH | 27114033 |
| HM_11:lacI_RBS(1)_DelH_rev | Sigma-Aldrich Chemie GmbH | VC00021`22003550 |
| AR07:TycC4dCom_short-BB_rev | Sigma-Aldrich Chemie GmbH | VC00021`22008078 |
| AR08:TycC4dCom_long-BB_rev | Sigma-Aldrich Chemie GmbH | VC00021`22008079 |
| AR02:c(TycC2)s_rev | Sigma-Aldrich Chemie GmbH | VC00021`22008073 |
| AR04:c(TycC2)l_rev | Sigma-Aldrich Chemie GmbH | VC00021`22008075 |
| AR05::l-Ind_fw | Sigma-Aldrich Chemie GmbH | VC00021`22008076 |
| AR03:s-Ind_fw | Sigma-Aldrich Chemie GmbH | VC00021`22008074 |
| AR01:sTycC4dC_fw | Sigma-Aldrich Chemie GmbH | VC00021`22008072 |
| AR06:lTycC4dC_fw | Sigma-Aldrich Chemie GmbH | VC00021`22008077 |
| RB92:entF-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020305 |
| RB93:tycA1-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020306 |
| RB93:tycC6-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020307 |
| RB94:delH4-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020308 |
| RB95:delH5-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020309 |
| RB96:plu2642-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020310 |
| RB97:plu2670-T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22020311 |
| RB107:tycC2_A_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098771 |
| RB106:tycC2_T3_rv | Sigma-Aldrich Chemie GmbH | VC00021`22098770 |
| RB99:plu2642_pRB23-plu_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098763 |
| RB100:plu2642_pRB23-plu_rv | Sigma-Aldrich Chemie GmbH | VC00021`22098764 |
| RB103:plu2642_valInd_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098767 |
| RB101:plu2642_A_rv | Sigma-Aldrich Chemie GmbH | VC00021`22098765 |
| RB105:tycC2_T3_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098769 |
| RB104:plu2642_valInd_rv | Sigma-Aldrich Chemie GmbH | VC00021`22098768 |
| RB102:indC_Ox_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098766 |
| RB108:tycC2_A_rv | Sigma-Aldrich Chemie GmbH | VC00021`22098772 |
| RB109:tycC2_valInd_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098773 |
| RB110:plu2642_TE_fw | Sigma-Aldrich Chemie GmbH | VC00021`22098774 |
| indC-BLAST | IDT | 65647038 |
| indC-T-Plum-BLAST | IDT | 65647039 |
| indC-T-BLAST | IDT | 65647040 |
| project5-BLAST | IDT | 65647041 |
| random1_indC | IDT | 65647042 |
| random2_project5-BLAST | IDT | 65647043 |
| random_indC-BLAST | IDT | 65647044 |
| RB90:indC-T-screen_fw | IDT | 65775415 |
| RB91:bpsA-T-screen_fw | IDT | 65775416 |
| PW38:CCDB_fwd | IDT | 65775417 |
| PW39:pSB1C3-CCDB_rev | IDT | 65775418 |
| PW40:CCDB-C(TycC2)_fwd | IDT | 65775419 |
| PW41:CCDB-C(TycC2)_rev | IDT | 65775420 |
| HM21_fw_lacI_BbsI_Xba | IDT | 65775426 |
| HM22_rev_RBS | IDT | 65775427 |
| HM23_fw_RBS | IDT | 65775428 |
| HM24_rev_BbsI_MfeI | IDT | 65775429 |
| PW42:pSB1C3-CCDB_rev_shorter | IDT | 65775430 |
| delH_f1_PacI_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f1_SalI_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f2_SalI_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f2_KpnI_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| AraCbb_KpnI_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| AraCbb_PacI_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| Screen_delH_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| Screen_delH_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| AraCbb_PacI_rev2 | Sigma-Aldrich Chemie GmbH | VC00021 |
| DelH_EcoRI_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| DelH_EcoRI_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| ygfG21C1 | Sigma-Aldrich Chemie GmbH | VC00021 |
| ygfG21C2 | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f1_fw_long | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f1_fw_short | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f1_PacI_fw_short | Sigma-Aldrich Chemie GmbH | VC00021 |
| delH_f1_fw_short2 | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK01:pLF03_integr_argK_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK02:pLF03_integr_pET21c_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK03:pLF03_integr_argK_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK04:pLF03_integr_argK_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK05:pLF03_integr_ygfG_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK06:pLF03_integr_ygfG_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK07:ygfG21C1 | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK08:ygfG21C2 | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_01_pSB4K5_DelA_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_11_DelAG_5_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_13_DelOP_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_15_DelL_mRFP_pSB4K5_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_02:DelAG1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_03:DelAG1_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_04:DelAG2_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_05:DelAG2_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_06:DelAG_3_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_07:DelAG_3_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_08:DelAG_4_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_09:DelAG_4_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_10:DelAG_5_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_12:DelOP_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_14:DelL_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_16:mRFP_pSB4K5_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB05:RBS1-BpsA_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB06:BpsA-RBS2_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB07:RBS2-svp_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB08:svp-pSB1C3_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB09:pSB1C3_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB10:pSB1C3-RBS1_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_11: DelAG_5_short_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_13: DelL_short_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_15: DelOP_short_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK_09:TycA_A1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK_10:TycA_A1_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK_19:TycB_A1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW_04:TycB_A1_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW_02:TycB_E1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW_03:TycB_E1_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB11:BpsA_Taka_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB12:BpsA_Taka_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB13:svp_Taka_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB14:svp_Taka_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB15:svp_Sanch_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB16:svp_Sanch_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB17:sfp_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB18:sfp_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB19:entD_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB20:entD_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM02_DelH_Gib1.1_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM03_DelH_Gib1.2_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM04_delH_Gib1.2_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM05_DelH_Gib2.1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM06_DelH_Gib2.1_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM07_DelH_Gib2.2_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM08_DelH_Gib2_end_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM09_AraC_RBS_Delh_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM10_RBS_lacZ_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_20: DelF_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_21: DelF_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_22: DelOP_short | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_24: DelAE | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_25: DelEF | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_26: DelFG_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK24:pccB_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK25:pccB_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| NI11:BpsA_rvN | Sigma-Aldrich Chemie GmbH | VC00021 |
| NI12:svp_fwN | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB21:pSB1C3_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB22:pSB1C3_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB27:indC-Plum_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB28:indC-Plum_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB35:sfp-Naka_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB36:sfp-Naka_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB37:Plum-extr_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB38:Plum-extr_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB43:Bsub-extr_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB44:Bsub-extr_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| FS_23: DelG_OP_long_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK12:TycC6_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK13:TycB1-dCom-dC_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK14:TycB1-TycC6_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK15:TycC6-TycB1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK16:TycA_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK17:TycA-dCom-TycC5_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK18:TycC5-TycA-dCom_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK19:TycB1-TycC5_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK20:TycC5-TycB1_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK21:pSB4K5-TycB1dComdC_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK22:pSB4K5-TycC6_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK23:pSB4K5-TycA_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW04:pSB4K5-TycC5dC_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW05:TycC5dC_fwd | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW06:TycC5dC-TycB1dCom_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW07:TycC5dC-TycB1dCom_fwd | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW08:C(TycB2)-TycAdCom_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW09:C(TycB2)-TycAdCom_fwd | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW10:TycAdCom-TycC6_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW11:TycAdCom-TycC6_fwd | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW12:TycAdE-TycC6_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| PW13:TycAdE-TycC6_fwd | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK26:BBa_I746200_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK27:BBa_I746200-BBa_B0029_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK28:pccB-BBa_XX_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK29:accA2-BBa_B0030_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK30:sfp-BBa_B0030-accA2_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK31:sfp_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK32:BBa_J04450-sfp_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK33:BBa_XX_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK34:pccB_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| IK35:BBa_J04450-pccB_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB23:bpsA-fus_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB24:bpsA-fus_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB25:svp-fus_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB26:svp-fus_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB29:svp-Svert_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB30:svp-Svert_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB31:Sc-indC-Schr_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB32:Sc-indC-Schr_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB33:entD-MG1655_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB34:entD-MG1655_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB39:Svert-extr_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB40:Svert-extr_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB41:MG1655-extr_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB42:MG1655-extr_rv | Sigma-Aldrich Chemie GmbH | VC00021 |
| RB45:Sc-indC-Schr_rvG | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM_11:lacI_RBS(1)_DelH_rev | Sigma-Aldrich Chemie GmbH | VC00021 |
| HM_12:DelH_RBS(1.2)_mRFP_fw | Sigma-Aldrich Chemie GmbH | VC00021 |
Table 7: Instruments
| Instrument | Type | Manufacturer |
|---|---|---|
| Table Centrifuge | Microfuge® 18 Centrifuge | Beckman CoulterTM |
| Table Centrifuge | Microfuge® 22R Centrifuge | Beckman CoulterTM |
| Centrifuge | Allegra X-12R | Beckman CoulterTM |
| Shaker | VORTEXGENIE 2 | Scientific Industries, SITM |
| Heating plate (magnet stirrer) | MR Hei-Standard | Heidolph |
| Heatblock | QBD4 | Grant |
| Heatblock (shakeing function) | Thermomixer comfort | Eppendorf |
| PCR-machine | MyCyclerTM thermo cycler | BioRad |
| PCR-machine | T100 Therma Cycler | BioRad |
| UV-chamber | Transluminator | Vilber Lourmat |
| Scale (fine) | PioneerTM PA114C | OHAUS |
| Scale | PioneerTM PA4101C | OHAUS |
| Fridge | KTP 1750 Premium | Liebherr |
| Freezer | GP 1366 Premium | Liebherr |
| Hood | Tischabzug | Wesemann® Laboreinrichtung |
| Draw-off pump | Vacuhand control | Vacubrand |
| Incubator | HT Multitron Version 2 | INFORS |
| Plate reader | ||
| Computer | Sun Microsystems | |
| UV/VIS Spectrometer | Ultrospec 3300 pro | Amersham Biosciences |
| Photometer | NanoDrop® ND-1000 Spectrophotometer | peQLab Biotechnologie GmbH |
| Photometer | NanoVue | General Electric |
| Ice Machine | MF22 | SCOTSMAN® |
| Gel electrophoresis chamber | Mupid®-One | Advance |
| Cell density meter | Ultrospec10 | Amersham Biosciences |
| pH-Meter | pH-Meter 765 Calimatic | Knick |
| Incubator | Heraeus | Thermo Scientific |
| Lyophilizer | Gefriertrocknungsanlage ALPHA 1-2 LD Plus Best. Nr. 101521 | Christ |
| Ultra sonification stick | Sonoplus Gm 2070 (2002) | Bandelin |
| Vacuum Manifold | Qiavac 24 Plus | QIAGEN |
| Gaskartusche Ventil CV470 | Burner | 13883 |
| NuPAGE(R) Novex 3-8% Tris-Acetate Gel 1 | SDS-Gel | Life Technologies GmbH |
Table 8: Mass spectrometry at neonate screening
| Instrument | Type | Manufacturer | Order number |
|---|---|---|---|
| micro filter plates | Multiscreen 96 well | Merck Millipore | MSHVN4550 |
| microtiter plates | 96 well Mikrotiter plates made of Polypropylen (F-Form) | Greiner | 655201 |
| microtiter plates locks | Micromats | ||
| aluminium foil | |||
| Mass spectrometer | Quattro Ultima (ESI-MS/MS) | Micromass (today: Waters) | |
| Methanol | (20837.320 METHANOL HIPERSOLV HPLC ISOCRATIC GRADE) |
Solution 1 (for MS sample preparation): Neo Gen stable isotope standard kit A und B (NSK-AB, EurIsotop)
1. One vial of amino acid standard (NSK-A) is taken up in 1 ml methanol/water (1:1 v/v) and solubilized for ca. 15 minutes in an ultra-sonification bath. Caution: make sure that the lid is closed well and not wetted by water of the ultra-sonification bath.
2. One vial of acylcarnitine standard (NSK-B) is taken up in 1ml of methanol and solubilized for ca. 15 minutes in the ultra-sonification bath.Caution: make sure that the lid is closed well and not wetted by water of the ultra-sonification bath.
Contents of both vials are transfered quantitatively with methanol in a 200 ml graduate flask.
The graduate flask is filled up to the calibration mark with methanol.
Standard solutions get a consecutive number. When two 200 ml graduate flasks are prepared simultaneously, contents of both flasks can be combined, if levels of both standards (old and new) coincide. Afterwards the whole solution will be labeled with one common number.
Stability of solution 1: 7 days
Solution 2 (for MS sample preparation): butanolic hydrochloric acid (3 N), water-free:
1. 900 ml butanol (p.A., Merck 1.01990.1000) are cooled in an ice bath at 0°C (magnetic stirrer).
The vessel has to be closed to avoid ice condensation of water.
2. 100 ml acetylchloride (p.A., Merck 1.00031.0250) are added dropwiese from a dropping funnel over a a period of 5 minutes while stirring constantly (protection goggles and gloves!).
Caution: make sure that no water or ice gets into the solution.
3. The solution is stirred for additional 10 minutes with closed lid on ice.
Stability of solution 2: 1 year
Solution 1 (for MS sample preparation):
Acetonitrile p.A./water (1:1 v/v) (83639.320DE ACETONITRIL HIPERSOLV SUPER GR REAG.PE)
Stability of solution 3: 1 year
Table 9: Lab materials
| Material | Name | Manufacturer |
|---|---|---|
| Bottle 50 ml, 100 ml, 200 ml, 500 ml, 1000 ml | ||
| Beaker 50 ml, 100 ml, 200 ml, 500 ml 1000 ml | ||
| Conical flask 500 ml, 1000 ml, 100 ml | ||
| Conical flask 300 ml | ||
| Single channel pipet | Pipetman® P2, P20, P200, P1000 | Gilson |
| Multichannel pipet | ||
| Multistep pipet | ||
| Pipet | ||
| 0.2 ml PCR Tube, Flat Cap, natural | I1402-8200 | Starlab GmbH |
| Microcentrifuge tubes | ||
| Centrifugetubes | ||
| PCR 8-strips | ||
| Gloves Nitril Gr. L | Starlab puderfr | Starlab |
| Gloves Nitril Gr. M | Starlab puderfr | Starlab |
| Gloves Nitril Gr. S | Starlab puderfr | Starlab |
| Filtertips | ||
| Inoculating loop | ||
| Disposal bags | ||
| Flasks | ||
| Plate | ||
| PETRI DISHES | P5731-500EA | Sigma-Aldrich Chemie GmbH |
| PETRI DISHES | N221.2 | Carl Roth GmbH & Co.KG |
| TipOne® Pipette Tip | S1110-1700 | Starlab GmbH |
| TipOne® Pipette Tip, 1000μl, Graduated | S1111-2721 | Starlab GmbH |
| neoBox-81 6er Set, je 1 x transparent, g | 22916 | |
| neoLab-Klebepunkte für Reaktionsgefäße | 19079 | |
| Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | |
| neoLab-Papierschere, 23 cm lang, gerade | 23272 | |
| TipOne® Pipette Tip, 200μl | S1110-1700 | Starlab GmbH |
| TipOne® Pipette Tip, 10μl, Graduated, Re | S1111-3700 | Starlab GmbH |
| PIPETTE, 5 ML | 606180 | Greiner bio-one GmbH |
| Bleiring mit Vinylbeschichtung, 57 mm In | 310161013 | NEOLAB GmbH |
| TipOne® Pipette Tip 10μl, Graduated, Rac | S1111-3800 | Starlab GmbH |
| Reaktionsgefäß,S.L.1,5ml,farbl. | 12682 | Eppendorf, Fisher Scientific GmbH |
| Reaktionsgefäß,S.L.,2ML, colorless | 12776 | Eppendorf |
| neoTape-Beschriftungsband, 13 mm, grau | 280126114 | NEOLAB GMBH |
| neoTape-Beschriftungsband, 25 mm, lachs | 280126229 | NEOLAB GMBH |
| 10ml Serological Pipette, Filter, Steril | E4860-1011 | Starlab GmbH |
| Gloves Latex + Alovera L | 14089 | |
| Gloves Latex + Alovera M | 14088 | |
| Gloves Latex + Alovera S | 14087 | |
| PH-stripes | WHAT10362000 | PANPEHA PLUS |
| 100 Run24Barcode | 20110099-100 | GATC Biotech AG |
| Röhrchen konisch, Polypropylen, 50 ml | 352070 | NEOLAB GMBH |
| Gene Pulser/MicroPulser Cuvettes, 0.1 cm | 165-2089 | Bio-Rad Laboratories GmbH |
| 0.2 ml 8-Tube Strips Without Caps, natur | TBS-0201 | Bio-Rad Laboratories GmbH |
| Rundbodenröhrchen, Polypropylen, 14 ml | 352059 | NEOLAB GMBH |
| Optical Flat 8-Cap Strips | TCS-0803 | Bio-Rad Laboratories GmbH |
| Impfschlingen 10 μl, blau, steril | 2900254437 | |
| corning serologische Pipetten 50ml | 14303 | |
| weighing dish 500 ST | 1884.1 | Carl Roth GmbH & Co.KG |
| filter paper | Z274836-1PAK | Sigma-Aldrich Chemie GmbH |
| inoculation loop 10µl | 2900254437 | NEOLAB GMBH |
Table 10: Plasmides
| Plasmide | Supplier | Catalog Number |
|---|---|---|
Table 11: Buffers
| NEBuffer Pack #4 (green) | New England Biolabs GmbH | B7004 S |
| NEBuffer Pack #1 (yellow) | New England Biolabs GmbH | B7001 S |
| NEBuffer Pack for T4 DNA Ligase | New England Biolabs GmbH | B0202 S |
| NEBuffer Pack #2 (blue) | New England Biolabs GmbH | B7002 S |
| NEBuffer Pack #3 (red) | New England Biolabs GmbH | B7003 S |
| TAE - Puffer (50X) für die Molekularbio | VWR International GmbH | A4686.1000 |
| Gel Loading Buffer | Sigma-Aldrich | G2526-5ML |
| Tris Acetate-EDTA buffer | Sigma-Aldrich | T9650-1L |
Table 12: other chemicals
| Isopropyl B-D-Thiogalactopyranoside 1 ST | Sigma-Aldrich Chemie GmbH | I5502-1G |
| Dimethyl Sulfoxide PCR Reagent | Sigma-Aldrich Chemie GmbH | D9170-1VL |
| Glycerol Sigma Grade | Sigma-Aldrich Chemie GmbH | G9012-100ML |
| 5-Bromo-4-Chloro-3-Indolyl B-D-*Galactop | Sigma-Aldrich Chemie GmbH | B4252-100MG |
| Bacteriological Agar | Sigma-Aldrich Chemie GmbH | A5306-250G |
| L-Plus-Arabinose Crystalline | Sigma-Aldrich Chemie GmbH | A3256-25G |
| Calciumchlorid Dihydrat | Th. Geyer GmbH & Co KG | SA/00223506/000500 |
| Malt extract from starch digestion | Sigma-Aldrich Chemie GmbH | M0383-100G |
| D(+)-Saccharose, ACS, for Micro-Biology | Sigma-Aldrich Chemie GmbH | 84100-1KG |
| Dimethyl Sulfoxide Plant cell culture*TE | D4540-100ML | |
| Sodium Hydroxide Anhydrous Pellets | Th. Geyer GmbH & Co.KG | SA/S5881/000500 |
| TRIZMA(R) Hydrochloride PH 3.5-5.0 | Sigma-Aldrich Chemie GmbH | T6666-50G |
| L-Glutamine 200 MM sterile | Sigma-Aldrich Chemie GmbH | G7513-20ML |
| Ethanol 96% Vergällt | Carl Roth GmbH & Co.KG | T171.3 |
| Propanol-2 | Sigma-Aldrich Chemie GmbH | 59309-1L |
| Natriumdodecylsulfat,SDS,99%, ultra pure | 13904 | |
| Gold(III)-chlorid | Carl Roth GmbH & Co.KG | 5624.1 |
| Pro-Leu | Sigma-Aldrich Chemie GmbH | P1130-1G |
| Salpetersäure 65% p.a. Iso | Carl Roth GmbH & Co.KG | X943.1 |
| mops, sodium | Sigma-Aldrich Chemie GmbH | M9024-25G |
| L-Glutamine | Sigma-Aldrich Chemie GmbH | G7513-100ML |
| Chelating Resin | Sigma-Aldrich Chemie GmbH | C7901-50G |
| potassium hydroxide in platellets | 6751.3 | |
| hydrochloric acid 37% | 4625.1 | |
| pyruvic acid | Sigma-Aldrich Chemie GmbH | 107360-25G |
| Fmoc-Orn(BOC)-OH 96.0 % | 47560-5G-F | |
| Glycerol >99,5% | Sigma-Aldrich Chemie GmbH | G9012-1L |
| Water Molecular Biology Reagent | Sigma-Aldrich Chemie GmbH | W4502-1L |
| Acetonitrile | Sigma-Aldrich Chemie GmbH | 34967-1L |
| ascorbic acid 99% | Sigma-Aldrich Chemie GmbH | A92902-100G |
Materials used by IGEM Heidelberg 2010
- good documented
https://2010.igem.org/Team:Heidelberg/Notebook/Material
