Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 20th August.html

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Target

Tuesday 20th August

Cloning of FdxA and FprA

Plasmid mini-prep of transformations
Nano dropped
  1. pETDuet: 59.1ng/ul
  2. pETDuet: 45.9ng/ul
  3. pJET Fdx: 54.2ng/ul
  4. pLS FRP: 19.7ng/ul

Digestion of FprA with NdeI and AvrII
Gel Clean up of FprA fragment
Digestion of pETDuet-1 with NdeI and AvrII
Digestion of FdxA with NcoI and HindIII
Digestion of pETDuet-1 NcoI and HindIII

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