"

From 2013.igem.org

Morning

• Tried to make clear agar using :

5g NaCl } 15g Tryptone } Makes 1 litre 15g Agar } 5g Soytone }

Tryptone, NaCl and soytone were added into a mixer with a magnetic stirrer. (was a yellow colour).

3g of agar were added to two 200ml durans. The Tryptone mix was then added to the durans, filling it up to 200ml.

Mixture was yellow, not colourless - the experiment was not successful. Durans put in the autoclave in the evening to let the agar set overnight.

Afternoon

• Transformation of competent cells