Exeter/3 July 2013

From 2013.igem.org

Making stock solutions of chloramphenicol, ampicillin and kanamycin

Chloramphenicol and kanamycin are TOXIC so need to be carefully weighed out in the fume hood. We are making 10ml stock solutions of all three antibiotics (none of the BioBricks we are using are in a tetramycin plasmid, so we have not made a stock solution of this antibiotic).

- Ampicillin, stock solution is 100mg/ml, so for 10ml we need 1000mg (1g)

- Chloramphenicol, stock solution is 35mg/ml, so we need 350mg

- Kanamycin, stock solution is 50mg/ml, so we need 500mg

Chloramphenicol must be dissolved in ethanol, the others can be dissolved in distilled water.

Weigh each antibiotic into an Eppendorf tube, then transfer into a 10ml Falcon tube. Add 10ml ethanol/water using a serological pippette, using a little of the ethanol/water to wash the last of the antibiotic from the Eppendorf. Seal each Falcon tube, and store in the freezer.

Making antibiotic LB agar plates