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Team:Tsinghua-E/Safety
From 2013.igem.org
Safety Form
This page answer the questions on the safety page. You can also downloadBasic safety Form of Tsinghua-E.pdf
Basic Safety Questions for iGEM2013
1.Please describe the chassis organism(s) you will be using for this project.
If you will be using more than one chassis organism, provide information on each of them:
|
Species |
Strain no/name |
Risk Group |
Risk group source link |
Disease risk to humans? If so, which disease? |
1 |
E.coli(DH5a) |
Biomed |
1 |
www.absa.org/riskgroups/bacteria search. php?genus=&species=coli |
Yes, May cause irritation to skin ,eyes and respiratory trait, may affect kidneys |
2 |
E.coli(BL21) |
Biomed |
1 |
www.absa.org/riskgroups/bacteria search. php?genus=&species=coli |
Yes, May cause irritation to skin ,eyes and respiratory trait, may affect kidneys |
3 |
E.coli(JM109) |
Biomed |
1 |
www.absa.org/riskgroups/bacteria search. php?genus=&species=coli |
Yes, May cause irritation to skin ,eyes and respiratory trait, may affect kidneys |
4 |
E.coli(JW3379) |
E.coli Kello Knockout Collection |
1 |
www.absa.org/riskgroups/bacteria search. php?genus=&species=coli |
Yes, May cause irritation to skin ,eyes and respiratory trait, may affect kidneys |
5 |
E.coli(JW3995) |
E.coli Kello Knockout Collection |
1 |
www.absa.org/riskgroups/bacteria search. php?genus=&species=coli |
Yes, May cause irritation to skin ,eyes and respiratory trait, may affect kidneys |
2. Highest Risk Group Listed:
ONLY 1
3. List and describe all new or modified coding regions you will be using in your project.
(If you use parts from the 2013 iGEM Distribution without modifying them, you do not need to list those parts.)
|
Part number |
Where did you get the physical DNA for this part (which lab, synthesis company,etc) |
What species does this part originally come from? |
What is the Risk Group of the species? |
What is the function of this part, in its parent species? |
1 |
pBAD_B0030_mutD-op_sfGFP |
cloned by PCR |
E.coli(BL21 DE3) |
2 |
DNA replication |
2 |
pBAD_B0032_mutD_op_sfGFP |
cloned by PCR |
E.coli(BL21 DE3) |
2 |
DNA replication |
3 |
pBAD_ori_mutD-op_sfGFP |
cloned by PCR |
E.coli(BL21 DE3) |
2 |
DNA replication |
4 |
pTrc99A_pBAD_trpsensor_malQ |
synthesized |
E.coli |
2 |
Confer maltose hydrolase activity |
5 |
pTrc99A_trpsensor_B0030_tetA |
synthesized |
E.coli |
2 |
Confer tryptophan dependent gene expression |
6 |
pTrc99A_trpsensor B0032_tetA |
synthesized |
E.coli |
2 |
Confer tryptophan dependent gene expression |
7 |
pTrc99A_trpsensor_ori_tetA |
synthesized |
E.coli |
2 |
Confer tryptophan dependent gene expression |
8 |
pTrc99A_trpsensor_lacZ |
synthesized |
E.coli |
2 |
Confer tryptophan dependent gene expression |
4. Do the biological materials used in your lab work pose any of the following risks? Please describe.
a. Risks to the safety and health of team members or others working in the lab?
1.We use ethidium bromide to detect nucleic acids in molecular biology laboratories and it is thought to act as mutagen because it intercalates double stranded DNA, which indicates that it can be toxic.
2.E.coli may have little effect on human health.
b. Risks to the safety and health of the general public, if released by design or by accident?
No.E.coli in our lab has nearly no difference with normal innocuous E.coli in nature.
c. Risks to the environment, if released by design or by accident?
No.E.coli in our lab has nearly no difference with normal innocuous E.coli in nature.
d. Risks to security through malicious misuse by individuals, groups, or countries?
The leakage of high -rate mutation bacteria may cause variety of problem to public and its result might be catastrophic. Unpredictable diseases caused by the bacteria we structured through mutation may lead to disastrous results,though the possibility of this extreme situation is tiny.
5.If your project moved from a small-scale lab study to become widely used as a commercial/industrial product,
what new risks might arise?(Consider the different categories of risks that are listed in parts a-d of the previous question.) Also, what risks might arise if the knowledge you generate or the methods you develop became widely available? (Note: This is meant to be a somewhat open-ended discussion question.)
The effect of mutation part may be enlarged to get more kinds of goal strains, so more uncertain factors will be added in.The possibility of new type of bacteria’s birth will increase sharply.
6. Does your project include any design features to address safety risks?
(For example: kill switches, auxotrophic chassis,etc.) Note that including such features is not mandatory to participate in iGEM, but many groups choose to include them.
Yes.All of bacteria that contain mutation part is auxotrophic.One of special genes of them has been knocked out so they can hardly survive in normal environment.In addition,our part of mutation stimulator will be destroyed in high temperature so as to get rid of spreading.
7. What safety training have you received (or plan to receive in the future)?
Provide a brief description, and a link to your institution’s safety training requirements, if available.
Training of extinguishing and protection,such as the manipulation of fire extinguisher and emergency shower in fire or biohazard.
8. Under what biosafety provisions will / do you work?
a. Please provide a link to your institution biosafety guidelines.
http://www.tsinghua.edu.cn/publish/sbc/202/2010/20101208153448737847778/20101208153448737847778_.html
b. Does your institution have an Institutional Biosafety Committee, or an equivalent group?
If yes, have you discussed your project with them? Describe any concerns they raised with your project, and any changes you made to your project plan based on their review.
We have the commitee but the discussion is not necessary because it’s a dally work to check the safety.
c. Does your country have national biosafety regulations or guidelines?
If so, please provide a link to these regulations or guidelines if possible.
http://www.gov.cn/zwgk/2005-5/23/content_256.htm
d. According to the WHO Biosafety Manual, what is the BioSafety Level rating of your lab?
(Check the summary table on page 3, and the fuller description that starts on page 9.) If your lab does not fit neatly into category 1, 2, 3, or 4, please describe its safety features.
1
e. What is the Risk Group of your chassis organism(s), as you stated in question 1?
If it does not match the BSL rating of your laboratory, please explain what additional safety measures you are taking.
Only 1
