Team:Groningen/Labwork/17 July 2013

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Mirjam
Restriction digest of the 1st and 3rd plate colonies are examined on gel. 5/7 colonies are correct transformations. To be completely sure if the transformation did work, a PCR for the promoter is made, using the colonies and the original construct as a backbone.


Miniprep for the samples of the 4th plate (they grew O/N). Restriction analysis with EcoRV.
Run gel of the restriction analysis.

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