Team:HokkaidoU Japan/Shuffling Kit/Primer Designer

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Maestro E.coli

Optimization Kit

Primer Designer for Maestro

1. First, you have to decide whether or not you conform to our default overhang set.

Yes, I conform to the default.

No, I design original overhangs.

2. Secondly, you hove to determine how many CDS are included in your target plasmid.

I have CDS(s) included.

3. Then, please select the region to where your fragment correspond in target plasmid.

1

2

3

4

5

6

7

8

9

Beginning of the fragment:

End of the fragment:

4. Okey, now we are ready to go. Enter your fragment's sequence, and press "Calculate"!

2. Please enter overhangs and fragment's sequence, and press "Calculate"

Forward

sequence

tm

Reverse

sequence

tm

5. Now, repeat previous step for remaining fragments included in the target plasmid.

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