Wet-lab verification
Although our focus in the development of our software is modeling and algorithms, as students majoring in biology, we find it necessary to testify the accuracy of our core ODEs and algorithms by wet-lab experiments. Instead of building a complex synthetic circuit and spending lots of time on it, it’s wiser to construct a handy,but elegant circuit.e
The host cell we used was BL21 (DE3) strain of E.coli, which had been genetically engineered to incorporate the gene for T7 RNA polymerase, the lactose promoter and operator in its genome.e
The plasmid we used was pET-28a. It contains a lacI gene, specific T7 promoter, kanamycin resistance gene. The coding gene, GFP, was cloned into the plasmid, located downstream of T7 promoter.e
![](https://static.igem.org/mediawiki/2013/4/44/SYSU-Wetlab11.jpg)