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Team:Hong Kong CUHK/futureapp
From 2013.igem.org
Revision as of 16:59, 28 October 2013 by Shengry0716 (Talk | contribs)
Human Practice
Future Application
Control enzymatic reactions
- Voltage switch allows fast and easy control of enzymatic reaction rate by electricity.
- We are working on demonstrating this with laccase and dioxygenase, and also BiFC
Bacterial Screening
- Utilize the BiFC
- Load cells onto multi-well plates
- Each well serves as a Pixel
Degradation of cellular proteins
- Utilize the Ubiquitin/26S proteasome system
- Linking the voltage switch with E1/E2/E3 protein
- Control degradation rate of cellular proteins by controlling ubiquitinylation rate
Study genes using protein complementation
- Split genes into two parts, and insert into our system
- Use voltage switch to control the split and refold of a gene.
- Possible tool for the screening of new protein complementation compatible proteins.
Study Membrane Potential of Organelles
- Target voltage switch system with BiFC onto the membrane of organelles interested
- Obtain fluorescent signal
- Estimate membrane potential
