Team:UCSF/Parts1

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BioBrick Parts Submitted to or Characterized in the Registry

Promoters

We characterized the pLAC and pTET inducible promoters and this information is available on the Experience page for each part.
1. Part: BBa_R0011, Link: pLAC
2. Part: BBa_R0040, Link: pTET

Guide RNAs (gRNA) for CRISPRi

We submitted 4 guide RNAs to the parts registry. The GFP and RFP gRNA's have been tested and shown to work in our experiments and in published results.
See the chart below for links to the parts registry pages for the RFP, GFP, XylE, and LacZ targeting gRNAs. Media References:
CDC sets threat levels for drug-resistant 'superbugs'
Agricultural Antibiotic Use Contributes To 'Super-Bugs' In Humans

SJTU-BioX-Shanghai 2012 iGEM Team Parts

Overview

We have characterized and submitted 42 BioBricks which could either be used directly or serve as a universal tool readily for potential scientific or engineering use.

Those BioBricks could be divided into 4 groups.

1.BioBricks in Basic Parts are all basic components of the whole project. They can be assembled to carry out different tasks.

2.BioBricks in Membrane Anchors are fusion membrane proteins which could either constitutively aggregate or aggregate under signal induction.

3.BioBricks in Fatty-Acid-Producing Membrane Accelerator are Membrane Anchors linked with enzymes involved in fatty acid synthesis.

4.BioBricks in Ligh-Sensing Membrane Rudder are Membrane Anchors linked with enzymes involved in Violacein synthesis and VVD protein.

<groupparts>iGEM012 SJTU-BioX-Shanghai</groupparts>


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