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Team:Shenzhen BGIC 0101/NewStandard
From 2013.igem.org
New Standard
Assembly Stategy Standard
Synthesized fragments to Biodevice Assembly
Different from the traditional bottom-up strategy, biobrick & 3A standard assembly, to assemble biobricks into a high-level biodevice, Genovo would assist users to directly design brand new chromosome denovo in silico and design its segmentation adding with adaptors, so that we can synthesize DNA fragments, and integrate them into megachunks by using "2k to 10k to 30k to whole" method and Gibson, Goldengate assembly, homologous recombination.
Chromosome Design Standard
After grap the information of pathway of interest, we can rewire genes' relation, replace genes with ortholog scoring higher, add human control upon genes' expression and experimentally synthesize this chromosome-like biodevice directly.
Design Operation Standard
Drag & drop biodevice Design
We can change the position, strand of genes in neochromsome, in a user-friendly way.
Data Transmission Standard
KGML is an XML presentation of KEGG pathway database, and rewrite relationships between genes use JavaScript (JS) language. In order to transfer XML be more readable for JS, we convert XML into JSON and transform data in more convenience way. We only reserve genes’ relationships and three kinds of subtype attribution, activation, inhibition and link. Through this data transmission standard, computer and users can directly and clearly understand the information. The diagram below is the data structure of our transmitted JSON format.
