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Main purpose

• Miniprep

Who was in the lab

Gosia, Henrike

Procedure

- miniprep of RFP in pZA21 and cycAX in pZA21

- made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21

- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment

- purified AMO for USER from gel

- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP)

- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp

- gel of colony PCR to check the inserts of last USER cloning -> empty, but the colonies that should have RFP are coloured red and one colony with supposed cycAX is coloured orange