Team:Heidelberg/Templates/MM week13p overview

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Goal

Vector map of the pIK1 plasmid.

Vector map of the pIK2 plasmid.

Back-up plan in case the genomic integration does not work. Two plasmids are to be constructed, pIK1 (containing BBa_I746200, pccB-accA, and sfp) and pIK2 (containing pccB-accA and sfp) in the pSB3C5 backbone. 6 fragments are to be amplified:

fragment ID	source	primers	size [kb]
f1	BBa_J04450 in pSB3C5	IK32+IK33	3
f2	BBa_I746200	IK26+IK27	2.3
f3	pccB-accA in pLF03	IK38+IK29	3.4
f4	sfp in BAP1	IK30+IK31	0.7
f5	BBa_J04450 in pSB3C5	IK32+IK35	3
f6	pccB-accA in pLF03	IK34+IK29	3.4