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From 2013.igem.org

Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.

Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.

Made a restriction digestion for the signal sequences.

Sander
Made a restriction digestion for silk strepF-R and silk F-strepR.

Inne
E. coli with the BBa_818000 backbone that had to grow overnight grew.

Sample	Composition	BBa_k818000 from	growth
1	4 mL LB, 8 uL Amp	iGEM 2012	Yes
2	4 mL LB, 8 uL Amp	iGEM 2012	Yes
3	4 mL, LB 8 uL Amp (negative control)	iGEM 2012	No
4	4 mL LB, 8 uL Amp, 4 uL IPTG	iGEM 2012	Yes
5	4 mL LB, 8 uL Amp, 4 uL IPTG	iGEM 2012	Yes
6	4 mL, 8uL Amp	iGEM 2013	Yes
7	4 mL, 8 uL Amp, 8uL IPTG	iGEM 2013	Yes
8	4 mL, 8 uL Amp (negative control)	iGEM 2013	No