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From 2013.igem.org

Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.

Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.

Made a restriction digestion for the signal sequences.

Sander
Made a restriction digestion for silk strepF-R and silk F-strepR.

Inne
E. coli with the BBa_818000 backbone that had to grow overnight grew.

Sample	Composition	BBa_k818000 from	growth
1	4 mL LB, 8 uL Amp	iGEM 2012	Yes
2	4 mL LB, 8 uL Amp	iGEM 2012	Yes
3	4 mL, LB 8 uL Amp (negative control)	iGEM 2012	No
4	4 mL LB, 8 uL Amp, 4 uL IPTG	iGEM 2012	Yes
5	4 mL LB, 8 uL Amp, 4 uL IPTG	iGEM 2012	Yes
6	4 mL, 8uL Amp	iGEM 2013	Yes
7	4 mL, 8 uL Amp, 8uL IPTG	iGEM 2013	Yes
8	4 mL, 8 uL Amp (negative control)	iGEM 2013	No

All suspensions were white. Decided to do a miniprep + digestion to see is plasmid was present. Samples 1 and 6 were chosen, 1 to check if renewed 2012 inoculation was a success and 6 to check if our stock had a the backbone. Upon down spinning the cells (first step of miniprep) a pinkish color was detected in the pellet of cell sample 6. This suggest that the BBa_k818000 backbone was in there after all. Sample 1 showed a less conclusive result. The cells weren't pure white, but also didn't display the same level of pinkness as sample 6.