Gel electrophoresis

Materials:

• Power supply
• Agarose gel
• Tray
• DNA samples
• DNA Ladder

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
The gel are run in TBE buffer 1x.