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From 2013.igem.org

Title

This is our new title(test).

Abstract

There exist various proteins which sense aromatic components and regulate the transcription of corresponding genes in prokaryotes, for example, Escherichia coli and Pseudomonas putida. However, naturally(natural) existing bio-sensors have various limitations including limited detection range, expression of leakage and low response ratio. This year our team designs a series of aromatic sensors and uses related enzymes in metabolic pathways to increase detectable aromatic compounds. We modify and characterize several existing biosensors. We also design a tunable band-pass gene circuit, which enables E.coli containing certain sensor respond to a specific inductor concentration. Combining the method of gradient dilution, we can measure the concentration of aromatic components in a convenient way. In the long run, these bio-sensors and promoters enrich the inducible device library in synthetic biology.

Project Abstract and Title

Project Title: YeasTherm®
Project Abstract: In Taiwan, fish farmers lose a large amount of fish because temperature falls dramatically when cold current comes in winter. Of course, fish farmers try to prevent fish from death dying; however, the current methods do not work well. Moreover, they cause damage to the environment. In 2013 iGEM competition, NTU-Taiwan team tries to make a bio-heating device. We transform the UCP (uncoupling protein) or AOX (alternative oxidase) gene into yeast and E.coli. UCP and AOX are thermogenins which can produce heat by interacting with the electron transport chain. By designing the gene circuit, we want to well control the power of the bio-heating device. In addition, we want to simulate the pound environment in reality by computer and the test results after using our device in low temperature.