lab 208

Main purpose

Who was in the lab

Procedure

Gel electrophoresis

Run 1% agarose gel on overnight PCR products.

Miniprep

Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using ethanol precipitation. Actually should have used the Midiprep kit from QIAgen. After ethanol precipitation continued with Midiprep kit step from step 9.

Results

Gels

1% agarose gel

• 1kb ladder
• Nir1 high
• Nir1 high
• Nir1 low
• Nir1 low
• broad band ladder
• 43
• 43
• Nir2 GC
• Nir2 GC
• neg for Nir
• AMO
• AMO
• neg for AMO
• 1 kb ladder

Conclusion

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