"
Exeter/29 July 2013
From 2013.igem.org
Revision as of 11:22, 6 August 2013 by FelicityHeath (Talk | contribs)
Liquid cultures
We made liquid cultures of:
Cph8 + RBS - K592018
B0015 - Terminator
K592022 - Cyan (w/RBS and promoter)
K864404 - Promoter, RBS and cyan
K592011 - Cyan pigment
S05058
Digest
We followed advice from a PhD student and did:
- Positive controls of
RFP cut with E and S
RFP cut with X and P
- Negative control of
Just water
The buffer and BSA were vortexed before use to make sure they were completely defrosted.
We got fresh purite water each time we needed it.
We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.
