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Exeter/29 July 2013
From 2013.igem.org
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Digest
We followed advice from a PhD student and did:
- Positive controls of:
- RFP cut with E and S
- RFP cut with X and P
- Negative control of:
- Just water
The buffer and BSA were vortexed before use to make sure they were completely defrosted.
We got fresh purite water each time we needed it.
We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.
