Team:Groningen/Labwork/6 August 2013

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Mirjam

Inoculated ΔDes and ΔCheY in minimal medium.
Inoculated CFP in LB medium with cm.
Mini prep analysis of Pdes/CheY. Figured out why this is not working.
Made a restriction digestion of YFP/GFP with XbaI and PstI.
Made a restriction digestion of CheY with BamHI and SpeI.

Claudio

The plates screened last Saturday do not show any positive result. None of the colonies screened are greenish.

The transformation of the positive candidate made last Saturday don't show any greenish colonies.