Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog

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Phage Purification July - August Notebook: August 1 - August 16 Daily Log

8/1/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.

8/2/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.

8/3/13

- E coli W3110 streak worked, but K12 did not survive. Thus, we tried to amplify K12 using liquid culture. For specifics, please consult 8.2 Modeling Phage Plaque Sizes - Experiment 1.

8/4/13

- Started E coli liquid culture over night

10mL of BL21 for spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.
2mL each of BL21, W3110, and B for phage viability and infection test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.

8/5/13

- Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1