Team:Groningen/Labwork/8 August 2013

Mirjam

Check of the plates of the restrictions made yesterday. One of the plates with CFP showed 1 colony. The other two plates (one IPTG) did not show any. The transformation of Pdes/CheY into the backbone failed, but the transformation of Pdes/CheY/eYFP showed a couple of colonies.
Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.
Made a new ligation reaction between Pdes and CheY.
Made a new ligation reaction between CFP and the backbone.
Made a new ligation reaction between Pdes/CheY and the backbone.
Transformation to E. coli of all the ligation reactions.

Claudio

Yesterday 2x SG plates in the presence of titanium are prepared.
2µl of Bacillus Subtilis overnight culture is spotted on titanium in the presence of 2x SG and without. The plates are incubated at 30°C.
Two of the Bacillus Subtilis complex colonies, which grew during the last few days on 2x SG, were transferred to titanium in the presence of SGG and without. The plates are incubated at 30°C.
100µl of the Bacillus Subtilis overnight culture was inoculated in 20 ml of SGG medium. The bottle is incubated at 30°C and pictures are taken every once in a while.