Team:Georgia State/project/overview

The primary purpose of our project is to modify the class of pGAPZα expression vectors according to iGEM standards to allow for expression and purification of proteins from an inducible yeast system. The glyceraldehyde-3-phosphate dehydrogenase (pGAP) promoter shuttle vector has been used in the methylotrophic yeast, Pichia pastoris, to express high levels of recombinant proteins (Waterham et al., 1997). The pGAPZα expression system allows for methanol-inducible expression of proteins with appropriate eukaryotic post-translational modifications. Additionally, the pGAPZα expression system allows for controlled secretion of a protein, making the purification of a protein relatively simple.