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From 2013.igem.org

Contents 1 Biolector Toxicity Experiment 1.1 Description 1.2 Results 1.2.1 All Results 1.2.2 Ammonium 1.2.3 Nitrate 1.2.4 Nitrite 1.3 Conclusions

Biolector Toxicity Experiment

Description

This project is to determine whether the non-gaseous intermediates (nitrite, nitrate) and ammonium are toxic to E. coli, and at what concentration. This experiment will use the biolector to grow E. coli in the presence of increasing concentrations of these ions and determine their OD and fluorescence.

Results

All Results

These panels show the response in biomass and fluorescence of GFP over time for varying concentrations of ions. The data has been baseline adjusted, etc[get details], by Chris' scripts [get name].

Ammonium

The horizontal facet shows concentrations in mM.

Nitrate

Nitrite

Conclusions

Concentrations of ammonium and nitrite were not sufficient to inhibit growth. Nitrate at concentrations of 25mM and greater inhibits both biomass and expression of GFP. Concentrations of nitrate at 5mM and greater show delayed expression of GFP and slower growth.