Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period10/Dailylog

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Phage Purification July - August Notebook: August 1 - August 16 Daily Log



Overview
March-April
May-June
July-August
September-October

8/2/13

- Performed the spot test in 7.29 Mutagen Concentration Test - Sixth Protocol.


8/5/13

- Because the top agar used in yesterday's spot was contaminated, we decided to redo this step. Also, because most of the spot tests went down to -6, we performed further dilution for the next testing to get a more accurate idea of phage concentration.

- Discussed modeling options: model phage plaque size against various variables including phage particle size, agar concentration, and bacterial concentration.

- Streaked E coli W3110 and K12 from frozen stock in preparation for the viability test in 8.2 Modeling Phage Plaque Sizes - Experiment 1.


8/7/13

- Performed a cesium chloride gradient purification on T7 mutated phage from the small phage team.

8.7 CsCl Gradient


8/4/13

- Started E coli liquid culture over night


8/5/13

- Performed Phage viability/infection test for 8.2 Modeling Phage Plaque Sizes - Experiment 1


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