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Team:Groningen/Labwork/16 August 2013
From 2013.igem.org
Mirjam
The colony of Pdes/CheY/GFP grown yesterday in LB medium is still white, so a mini prep is done. To determine if the construct is correct, a restriction analysis is done with EcoRI and PstI, and EcoRI and XbaI. The gel shows again a band at 1100 bp, which is incorrect correlated to the respected heights (300-300-700).Two possible reasons:
1. Pdes is not inserted into the construct
2. Due to our XbaI-SpeI overlap, CheY is removed from the construct.
iGEM 2013 Groningen
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