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Team:Paris Saclay/Notebook/August/12
From 2013.igem.org
Contents |
Notebook : August 12
Lab work
A - Aerobic/Anaerobic regulation system
Obtaining Pfnr_RBS-LacZ-Term in PSB1C3
1 - Digestion of Bba_K1155000 by SpeI and PstI, Bba_K1155007 and Bba_K1155003 by XBaI and PstI
Anaïs, Nadia, XiaoJing
- Bba_K1155000 :
- Buffer FD : 5µL
- H2O : 38µL
- DNA : 5µL
- SpeI : 1µL
- PstI : 1µL
- Bba_K1155007 :
- Buffer FD : 5µL
- H2O : 23µL
- DNA : 20µL
- XBal : 1µL
- PstI : 1µL
- Bba_K1155003 :
- Buffer FD : 5µL
- H2O : 33µL
- DNA : 10µL
- XBal : 1µL
- PstI : 1µL
2 - Electrophoresis of digestion of Bba_K1155000 by SpeI and PstI, Bba_K1155007 and Bba_K1155003 by XBalI and PstI
Nadia
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Expected sizes :
- RBS_LacZ_Term : 3500 kb
- RBS_AmilCP_Term : ...
- PSB1C3 : ...
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We can't see any band for Bba_K1155000 digestion. The digestion failed. We will do it again. We obtain RBS-LacZ-Term and RBS-AmilCP-Term fragments. The digestion was good. We can now purify it. |
3 - Digestion of Bba_K1155000 by SpeI and PstI
Anaïs, Nadia
- Buffer FD : 5µL
- H2O : 38µL
- DNA : 5µL
- SpeI : 1µL
- PstI : 1µL
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : RBS-BphR2 part I, BphR2 part I, BphR2 part II, RBS-FNR part I, FNR part I and FNR part II
Damir
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