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Team:Wisconsin-Madison/protocol
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Protocol
Expression and Purification of Enzymes
Solutions
Resuspension Buffer
- 400 mM NaCl
- 50 mM NaHPO4
- 2.5% (v/v) glycerol
- 15 mM Imidazole
- 0.05% Triton X-100
- pH 8.0
