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• PCR was run
• transformation of [http://parts.igem.org/Part:BBa_I0500 Biobrick I0500] on Amp instead of Kan
• transformation of [http://parts.igem.org/Part:BBa_K564012 Biobrick K564012] on CAM
• we used two different batches of competent cells and 200 ul of cells instead of 50ul
• we incubated on LB rather than SOC

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