Team:Groningen/Modelling/22 August 2013

did a plasmid isolation of the BBa_J04450 submission backbone. created a large quantity of backbone stock placed together in a bag in the freezer. Leave it all to the modellers 8-) Today we implemented the simulated annealing algorithm for approximating the chemotaxis parameters. It failed horibly. As an error function we used the distance between TM0, TD0, VP and AP concentrations when 0 attractant and 10 micro mol attractant was present, the maximum value of YP, and the distance between YP with concentrations of 0 and 10 micromol attractant present. It strived to maximize YP with 10 micromol present, minimize the distance between YP equillibria with 0 and 10 micro mol present, and to maximize the distance between AP, VP, TD0 and TM0 with 0 and 10 micromol present. We expect it failed because of the sensitivity of the model, the sheer volume of the search space, and variables in the error function that, to some extent, strive to contradict one another. Tomorrow we will try again.