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From 2013.igem.org

NanoDrop data of the miniprepped, defrosted DNA:

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

• OmpF A - 20.1 ng/ul
• OmpF B - 9.3ng/ul
• B0034 - 26.7ng/ul
• RFP - 198ng/ul

The concentration for OmpF B was too low, so we continued on to the digestion with only OmpF A. The concentration for RFP was too high, so we diluted it.

• RFP (dilution) - 50.4ng/ul