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Lab 208

Main purpose

• Biobrick preparation

Who was in the lab

Henrike, Kristian, Julia

Procedure

Introduce endings for USER cloning in pSB1C3

In order to introduce our constructs in the biobrick vector we need to introduce special endings that are necessary for the USER cloning reaction. The table shows the composition of each reaction:

Results

Gel pictures

Ran gels on yesterday's PCRs to create psB1C3 with USER endings.

Small gel:

• 1 kb ladder
• 2%, pur
• 4%, pur
• 5%, pur
• 4%, 1uL, pur
• 4%, 2uL, pur
• 1M, pur
• 2%, HQ
• 4%, HQ
• 5%, HQ
• 4%, 1uL, HQ
• 4%, 2uL, HQ
• 1M HQ
• 1 kb ladder (ladder was almost empty so I put another one in the next lane)
• 1 kb ladder

big gel (samples from touch down PCR):

• 1 kb ladder
• GC, HQ
• 2% HQ
• 4% HQ
• 5% HQ
• 4%, 1uL, HQ
• 4%, 2uL, HQ
• 1M HQ
• GC pur
• 2%, pur
• 4%, pur
• 5%, pur
• 4%, 1uL, pur
• 4%, 2uL, pur
• 1M pur
• neg
• 1 kb ladder

Conclusion

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