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To create a means of communication from cell to machine, we chose the red fluorescence protein KillerRed as a reporter protein. The first condition that our device needs to fulfill is to record light intensity. Then it needs to generate fluorescence thank to a light source and a couple of excitation and emission filters. Firstly we will explain the choice of the different components, then the several experiences we did to find the most accurate parameters for each part of the device – the photodiode, Arduino and the optic.
To record light intensity, we were inspired by the E. glometer of the Cambridge team of iGEM 2010.
The E. glometer of Cambrige team (iGEM 2010)
Device built by Cambrige team in 2010 to measure the light intensity of their LuxBrick
Source:https://2010.igem.org/Team:Cambridge/Tools/Eglometer
TALK'E coli: C2M part
on the left: the real device, on the right: functional schematic
The light from the LED lamp goes through the green excitation filter and illuminate the sample thanks to a dichroic mirror. Then the red fluorescent protein is now excited and re-emits red light that goes through a lens that concentrate it on the photodiode.
