lab 208

Main purpose

Who was in the lab

Procedure

PCR to amplify HAO and cycAX USER fragments

Results

Gel

• 1 kb ladder
• psB1C3 for Nir, 2% DMSO
• psB1C3 for Nir, 2% DMSO, 1 uL 50mM MgCl2
• psB1C3 for Nir, 4% DMSO
• psB1C3 for Nir, 4% DMSO, 1 uL 50mM MgCl2
• psB1C3 for Nir, 1M Betaine
• purification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?)
• purification of HAO fragment (escaped from well -> Ethanol?)
• purification of AMO fragment (escaped from well -> Ethanol?)
• purification of psB1C3 USER fragment to make Biobricks (used more loading buffer)
• purification of HAO fragment (used more loading buffer)
• purification of AMO fragment (used more loading buffer)
• purification of pZA21::ara(tight)
• purification of pZA21::ara(tight) with endings for Nir
• 1 kb ladder

Conclusion

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