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• Team:Macquarie Australia/Notebook2
  ...esults indicated that all the genes had similar intensities to the control plasmid and vectors, except for ChlM, which was slightly lower. ...ed. The results indicated that the gene was successfully inserted into the plasmid vector.
  38 KB (6,401 words) - 02:25, 28 September 2013
• Team:Goettingen/NoteBook w5
  <p class="MsoNormal"><span lang="EN-US">15 µl part 7 plasmid DNA (1 µg)</span></p> <p class="MsoNormal"><span lang="EN-US">5 µl GFP-Term C1 plasmid DNA (1 µg)</span></p>
  121 KB (16,849 words) - 19:59, 30 September 2013
• Team:Freiburg/Project/effector
  ...ps://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a></b>, a plasmid coding for the required RNAs. By using several RNAs mutliple targeting is a ...ecting our <a id="link" href="http://parts.igem.org/Part:BBa_K1150034">RNA plasmid</a> which codes for the desired crRNA, dCas9-VP16 specifically binds to the
  45 KB (6,905 words) - 03:49, 29 October 2013
• Team:Hong Kong HKUST/notebook/mod3
  Plasmid extraction for pCMV/myc/mito <br /> Digestion of pCMV plasmid extraction product with PstI-HF and NotI-HF <br />
  26 KB (4,151 words) - 23:14, 27 September 2013
• Team:IIT Delhi/Notebook
  ...estriction enzymes present in the lab are usable, we digested the isolated plasmid with the two enzymes separately and ran them on the gel. Following was the ... plasmid DNA. This plasmid was discarded and fresh colonies inoculated for plasmid isolation.<br>
  18 KB (2,726 words) - 03:21, 28 September 2013
• Team:ITU MOBGAM Turkey/labdiary
  Both manual and Roshe plasmid isolation kit was applied to isolate the Terminator. However, low concentra <p>Plasmid isolation was made to RBS and T7 Promoter by the kit and manually.
  33 KB (5,137 words) - 20:58, 4 October 2013
• Team:Frankfurt/Notebook/Protocol
  ==Plasmid Preparation== __NOEDITSECTION__ ===Plasmid Preparation from ''E.coli'' (Mini Preparation)=== __NOEDITSECTION__
  12 KB (1,850 words) - 02:00, 5 October 2013
• Team:Heidelberg/Templates/Materials list
  | Plasmid Plus Maxi Kit (25) || QIAGEN || 12963 | Plasmid Plus Midi Kit (25) || QIAGEN || 12943
  95 KB (12,744 words) - 07:35, 15 January 2014
• Andrew Crouch
  *Started a 1 L culture of EcNR2 with our plasmid with the pLtetO promoter in LB min + kanamycin + arabinose + ATC *Started an EcNR2 with our plasmid with pLtetO culture of 3ml LB with 3ul Kan
  32 KB (5,157 words) - 13:43, 11 October 2013
• Team:SYSU-China/Notebook/Labnotes
  1. DNA extraction techniques: Plasmid extraction, DNA clean up(extraction from PCR or restriction endonuclease di Contents: First plasmid construction：pCDNA3.0-PGK-BSD
  26 KB (4,093 words) - 04:21, 28 September 2013
• Team:Marburg/Notebook:July
  <span class="aim-desc">Check correct assembly of the plasmid for antibody production</span> <li>1 µl Plasmid (pSB1A3-iBB10+iBB9, pSB1A3-iBB11+iBB9, pSB1A3-iBB12+iBB9 and pSB1A3-iBB13+i
  43 KB (5,715 words) - 11:15, 27 October 2013
• Team:Warsaw/Genetic lab journal
  Isolate plasmid DNA by alkaline lysis. Quality checked with spectrophotomeric analysis with ... with mini-prep kit. Digest and ligation of construct: J23100 and B0034 on plasmid pSB1C3.
  14 KB (2,179 words) - 17:28, 4 October 2013
• Team:NTU-Taida/Notebook/Journal/August
  #Ligation the three tubes overnight (plasmid undergo blue-white selection) ===Plasmid extraction===
  14 KB (2,061 words) - 21:25, 27 September 2013
• Team:INSA Toulouse/contenu/lab practice/notebook/protocols/backbone
  <h3 class="title3">Plasmid Backbones</h3> ...<i>This protocol was developed by Tom Knight. Samples of standard Registry plasmid backbones prepared using this method are sent out in the DNA Distribution k
  12 KB (1,848 words) - 09:55, 9 September 2013
• Team:UCSF/Project/Conjugation/Data
  ... conjugative genes integrated into the chromosome, and a small conjugative plasmid containing the origin sequence for conjugative transfer, pARO190. We were t ...es and selected for target strain cells that have received the conjugative plasmid, which we call “transconjugates”.
  12 KB (1,757 words) - 02:00, 29 October 2013
• Team:Peking/Project/BandpassFilter
  ...C3 as backbone. The regulator plasmid was co-transformed with the reporter plasmid, and then the activation curve was measured. <b>(Fig. 13)</b> ...id promoter Phyb and report gene sfGFP. The regulator plasmid and reporter plasmid use pSB4K5 and pSB1C3 as their backbone, respectively.</p>
  43 KB (6,197 words) - 20:39, 28 October 2013
• Team:Goettingen/NoteBook w4
  ...span lang="EN-US">the other clones (<span class="SpellE">linearized</span> plasmid part 7 and <span class="SpellE">linearized</span> plasmid/5 µl<o:p></o:p></span></p>
  212 KB (28,088 words) - 19:56, 30 September 2013
• Team:SJTU-BioX-Shanghai/Notebook/Lab log/September
  ===Construct t7-lac inducible dCas9,pcyA and Ho1 plasmid=== Digest T7-pcyA-pRSFDUET plasmid and PCR product and then purify.
  3 KB (417 words) - 04:17, 28 September 2013
• Team:UCL/BacterialLabs
  ....org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out. ...CL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="
  53 KB (9,305 words) - 23:54, 1 October 2013
• Team:UCL/MammalianLabs
  ....org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out. ...CL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="
  53 KB (9,308 words) - 09:18, 2 October 2013