User contributions
From 2013.igem.org
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- 13:39, 3 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Tuesday, July 2nd)
- 22:32, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Transformation of E. coli XL1-blue with ligation product F164 + F131 (SERK-SigP_SpyCatcher_Strep-tag-II-TEV-site-linker_SERK-TMD_8AAlinker_GFPmut1 in pSB1C3+ IRES), F165 + F131 (SERK-SigP_SpyCatcher_Strep-tag-II-TEV-site-linker_SERK-TMD_8AAli)
- 22:31, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Ligation of F164 + F131 (SERK-SigP_SpyCatcher_Strep-tag-II-TEV-site-linker_SERK-TMD_8AAlinker_GFPmut1 in pSB1C3+ IRES) and F165 + F131 (SERK-SigP_SpyCatcher_Strep-tag-II-TEV-site-linker_SERK-TMD_8AAlinker_GFPmut1 in pSB1C3 + IRES))
- 20:28, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Transformation of E. coli XL1-blue with ligation product F162+F42 (pActin+pSB1C3, ligation over night at 16 °C)
- 20:28, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Transformation of E. coli XL1-blue with ligation product F162+F42 (pActin+pSB1C3, ligation batch volume 10 µl, reaction performed at room temperature) and with F159+F163 (stress inducible promoter+RFP+t35S+NPT in pSB1C3))
- 20:27, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Transformation of E. coli XL1-blue with ligation product F162+F42 (pActin+pSB1C3, ligation over night at 16 °C)
- 20:26, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Tuesday, July 2nd)
- 20:20, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Tuesday, July 2nd)
- 20:07, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Transformation of E. coli XL1-blue with ligation product F162+F42 (pActin+pSB1C3, ligation batch volume 10 µl, reaction performed at room temperature)
- 20:05, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Ligation of F162 (pActin) + F42(pSB1C3 backbone))
- 19:58, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Tuesday, July 2nd)
- 14:54, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Ligation of F162 (pActin) + F42(pSB1C3 backbone))
- 14:53, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Picking of F123 + F152, F157 + F131, F158 + F131, F159 + F151, F150 + F160 and F123 + F161)
- 14:50, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Picking of F123 + F152, F157 + F131, F158 + F131, F159 + F151, F150 + F160 and F123 + F161)
- 14:49, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Picking of F123 + F152, F157 + F131, F158 + F131, F159 + F151, F150 + F160 and F123 + F161)
- 13:54, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Transformation of E. coli XL1-blue with ligation product F162+F42 (pActin+pSB1C3, ligation for 1 h at room temperature)
- 13:52, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Tuesday, July 2nd)
- 13:51, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Ligation of FF161 (pActin) + F42(pSB1C3 backbone))
- 13:42, 2 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Monday, July 1st)
- 20:27, 1 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Preparative digestion and gelelectrophoresis of F155 (pActin PCR product) and P393 (Stress inducible Promoter))
- 20:23, 1 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Everyday I'm swaffeling)
- 20:22, 1 July 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Everyday I'm swaffeling)
- 20:19, 1 July 2013 (diff | hist) N File:TUM13 20130701 prep Verd F155 P393.png (top)
- 20:42, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and F154 (FluA)
- 20:38, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and F154 (FluA)
- 20:38, 30 June 2013 (diff | hist) N File:TUM13 20130630 prepVerd P404 EcoRI.XbaI P395 NgoMIV.SpeI F154 XbaI.AgeI.png (top)
- 20:37, 30 June 2013 (diff | hist) N File:TUM13 20130630 prepVerd P367 XbaI.NgoMIV P416 SpeI.PstI P418 SpeI.PstI.png (top)
- 20:36, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and F154 (FluA)
- 20:34, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and P418 (FluA)
- 20:31, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and P418 (FluA)
- 20:28, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Preparative digestion and gelelectrophoresis of P367 (NLS+thermonuclease), P416 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6), P418 (PhyB_36AAlinker_C-TEV_IRES_N-TEV_36AAlinker_PIF3/6 ), P404 (t35S_npt-casette), P395 (SpyTag)and P418 (FluA)
- 20:25, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→PCR of P143 (pLuc-Actin, Amplification of pActin))
- 19:27, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→PCR of P143 (pLuc-Actin, Amplification of pActin))
- 19:10, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→PCR of P143 (pLuc-Actin, Amplification of pActin))
- 19:08, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→PCR of P143 (pLuc-Actin, Amplification of pActin))
- 19:07, 30 June 2013 (diff | hist) N File:TUM13 20130630 anal gel PCR P143 pActin.png (top)
- 19:04, 30 June 2013 (diff | hist) N File:TUM13 20130630 anal gel PCR P143 pActin.Tif (top)
- 15:21, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Sunday, June 30th)
- 15:19, 30 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Sunday, June 30th)
- 13:01, 27 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 12:57, 27 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (/* Sequencing of P367 (NLS-Thermonuclease), P376 (AlcR QC II), P374 (npt-casette QC II), P348 (Laccase), P350 (SERK-SigP_EreB_Strep-tag-TEV-site-linker_SERK-TMD_8AAlinker_GFPmut1), P352 (SERK-SigP_SpyCatcher_Strep-tag-TEV-site-linker_SERK-TMD_8AAlink)
- 10:21, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 10:20, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 10:19, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 10:19, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 10:18, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Wednesday, June 26th)
- 10:17, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Miniprep of overnight cultures of transformated E. coli XL1 blue with P270, F113+F131, F109+F130, P308, F115+F131, F92+F130, F115+F131, P272, P366, lucBB)
- 10:13, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Sequencing of P378 Proteinphosphatase 1)
- 10:05, 26 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Sequencing of P378 Proteinphosphatase 1)
- 16:30, 6 June 2013 (diff | hist) Team:TU-Munich/Notebook/Labjournal (→Ligation of F42+F51)
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