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Team:Groningen/Labwork/5 September 2013
From 2013.igem.org
Claudio
The plates (pSB1C3-S1-S5 and pSB1C3-S2-S5) show a lot of colonies.ColonyPCR is performed on 4 colonies per plate (total of 8 colonies) beside one positive control (pSB1C3-S1). The primers used are VF2 and VR (annealing temperature 58°C).
PCR was performed on S3 and S9 using primer S34910.
PCR was performed on S6, S13 and S14 using primer S16.
PCR was performed on S16 using primer true-S16.
The annealing temperature tested for all of them is 58°C.
iGEM 2013 Groningen
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