Team:DTU-Denmark/Methods

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Methods

Contents

1 PCR
2 USER Cloning
3 Plasmid isolation
4 Determining concentration of nitrogen compounds
5 Other

PCR

USER Cloning

USER Cloning and Transformation

Plasmid isolation

Plasmid isolation by ethanol precipitation

Determining concentration of nitrogen compounds

Other

Preparation of chemically competent cells

Sparging with N₂

Modified DM minimal medium

Calibration of Electrodes

Ammonium colorimetric measurements

Nitrate colorimetric measurements

Nitrite colorimetric measurements

Rehydrating Primers

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