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• iGEM 2013

Contents 1 September 1.1 9/4/13 1.2 9/6/13 1.3 9/9/13 1.4 9/11/13 1.5 9/13/13

September

9/4/13

Results from 8/28/13: Fractions 20 and 21 produced clearage on 10^0, and many small plaques at 10^-2. #4 had one plaque on 10^-2, and #1 had one plaque on 10^0.

Today we infected 0.5 mL of E. coli with: 100 uL of #1 10^0, 100 uL of #4 10^0, 10 uL of #20 10^-2, 10 uL of #21 10^-2, and for controls we did 10 uL of T4 wild type 10^-6 and 1 uL of T4 10^-7. We also plated a lawn of E. coli as a control.

BDM KW

9/6/13

Results from 9/2:

In general, the plaques for the mutagenized phage seem to be smaller than the pre-mutagenized phage. From this it would appear that we have large phage! But, it's important to make sure that we have a stable phage, so from here we will be picking the small plaques from the mutant plate and plating them out so we can observe if there is a steady result of small plaques. BDM,KW

9/9/13

9/11/13

9/13/13