Team:Groningen/Labwork/10 September 2013

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Mirjam

Genomic DNA extraction for ΔCheY and ΔDes.

Claudio

pSB1C3-S1-S5-S13 and pSB1C3-S2-S5-S14 plates showed colonies.
ColonyPCR on 5 colonies per plate was performed using VF2 and VR primers (annealing temperature 58°C).
The samples were checked on agarose gel 0.8% beside the positive control (pSB1C3-S1-S5).